The neuropharmacology and Clinical Laboratories of the Children's Hospital Medical Center in Cincinnati
have recently joined to develop and offer a new test profile for assessing coenzyme Q10 status.This unique profile includes the concentrations of total,
oxidized, and reduced forms of this essential cofactor, plus the total Q10 concentration indexed with total cholesterol.
Coenzyme Q10 (also called CoQ or ubiquinone) is an endogenous cofactor which is
essential for mitochondrial function and cellular energy production. It also serves as an
important antioxidant, protecting membranes from oxidative injury, and conserving and
regenerating vitamin E. A new method for measuring the reduced (or antioxidant) and oxidized forms of coenzyme Q10 has been developed in the CHMC neuropharmacology laboratory (see Clinical Chemistry 2001;47(2)256-265
Because coenzyme Q10 may be
depleted in certain acute and chromic disease states, the ratio of reduced to total Q10
concentration is also a useful biomarker of oxidative stress. Patients who have coenzyme
Q10 deficiency also have increased risk of mitochondrial and cellular injury from excess
production of free radicals. Certain drugs and malnourished states may also tend to
deplete the body's coenzyme Q10 reserves.
Measurement of coenzyme Q10
concentrations in plasma can assist clinicians in detecting coenzyme Q10 deficiency
states, and serve as a guide for dosing when oral supplementation is indicated.
Because significant inter-product variability in the absorption and bioavailability of coenzyme
Q10 has been reported with over-the-counter (OTC) Q10 preparations, the Coenzyme
Q10 Profile will also assist in verifying the extent of absorption of Q10 from those
Included in the Coenzyme Q10 Profile are the oxidized, reduced, and total Q10
concentrations. Also the fraction of the reduced Q10 in the total concentration, and the
Q10: Total Cholesterol Index (or QCI0) are reported. Reference intervals and comments
are also provided with each report.Potential indications for testing coenzyme Q10 status include:
- Suspected coenzyme Q10 deficiency secondary to inheritance (rare) or other mitochondrial disease
- Neurodegenerative diseases
, e.g. Parkinson's, Alzheimer's, Muscular Dystrophy
including Duchenne Muscular Dystrophy (DMD)
, and Amyotrophic Lateral Sclerosis (ALS)
- Metabolic disease
- Presurgical screening
for patients who have pre-existing coenzyme Q10 deficiency or
who may have increased risk of ischemia-reperfusion injury
- Prior treatment with HMG-CoA reductase inhibitors ("statin" drugs
) or other drugs
which may lower coenzyme Q10 levels
- Gastrointestinal malabsorption
- Assessment of coenzyme Q10 dosing adequacy
The Coenzyme Q10 Profile requires a minimum of 2 mL of heparinized blood (green to
vacutainer tube), which must be placed on ice immediately and sent to the CCHMC
Clinical Lab Administration Office for next morning delivery. Please call the CCHMC Clinical
Lab Administration Office at (513) 636-7328 in advance for billing, ordering,
shipping, and specimen collection information.
For technical questions about Coenzyme Q10 testing
, please contact Dr. Peter Tang, Ph.D., Pathology and Laboratory Medicine at (513) 636-3357.
For additional information on the Coenzyme Q10 Profile
, please contact Dr. Michael Miles, Pharm.D., CCHMC
Division of Child Neurology at (513) 636-7871.
Michael V. Miles, PharmD
Prof. Of Clinical Pediatrics and Neurology
Director, Clinical Neuropharmacology Laboratory
Children's Hospital Medical Center
3333 Burnet Ave.
Cincinnati, OH 45229
Phone: 513/636-7871 Fax: 513/636-3980
Need More Information?
For more information on what CoQ10 is and how it works, "click" on the "What is CoQ10?"
button. For more information on Q-Gel® CoQ10 specifically, "click" on the "Q-Gel® CoQ10"
button. Both buttons are located on upper left hand side of the screen (you may have to "scroll up" to see them